Zebrafish Eye, Light Sheet Imaging, 2 color, Time Lapse
Duration: 49 secs
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| Description: |
29h time lapse movie of a developing transgenic zebrafish retina, starting ~ 30hpf (hours post fertilisatisation). The researchers aim to visualise the whole process of embryonic retinal differentiation by labelling each of the 5 neuronal types with a distinct colour.
In this movie, two transgenes were used: Ath5:gapRFP, where the Ath5 promotor drives the expression of membrane tagged RFP in retinal ganglion cells as well as in photoreceptors, horizontal and amacrine cells; and Ptf1a:cytGFP, where the Ptf1a promotor drives the expression of cytoplasmic GFP in amacrine and horizontal cells. The only cell type that remains to be labeled here are the bipolar cells. At the start of the movie, many of the cells are already labelled with RFP, whereas the GFP expression has just started in a few cells. As the retina develops, a wave of differentiation sweeps across the retina, as cells start to differentiate and migrate to their appropriate position. After 12 hours, the retinal ganglion cell layer is already established and we can already observe some retinal ganglion cells axons exiting the eye and projecting towards the brain. At the same time, the amacrine cell layer has also been established and towards the end of the movie we can see some Ptf1a+ horizontal cells migrating towards the edge of the retina, to establish the horizontal cell layer. |
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| Created: | 2015-05-18 16:02 | ||||
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| Collection: | CAIC - Cambridge Advanced Imaging Centre | ||||
| Publisher: | University of Cambridge | ||||
| Copyright: | Dr M.O. Lenz | ||||
| Language: | eng (English) | ||||
| Distribution: |
World
(downloadable)
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| Explicit content: | No | ||||
| Aspect Ratio: | 4:3 | ||||
| Screencast: | No | ||||
| Bumper: | minimal black | ||||
| Trailer: | minimal black | ||||
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| iPod Video | 480x360 | 398.2 kbits/sec | 2.38 MB | View | Download | |
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